Identification of proteins in blood nucleoprotein complexes
Autor: | D. S. Serdukov, S. N. Tamkovich, O. S. Tutanov, T. G. Duzhak, Pavel P. Laktionov |
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Rok vydání: | 2015 |
Předmět: | |
Zdroj: | Russian Journal of Bioorganic Chemistry. 41:617-625 |
ISSN: | 1608-330X 1068-1620 |
DOI: | 10.1134/s1068162015060163 |
Popis: | Circulating nucleoprotein complexes were isolated-from blood plasma by affinity chromatography using immobilized polyclonal anti-histone antibodies. It was found, that the main part of DNA from histone-contained nucleoprotein complexes have size 170-180 b.p., in blood of breast cancer patients DNA with size 170-180 b.p. and DNA more then 6 k.b.p. are presented in equal quantity. Proteins from circulating nucleoprotein complexes were identified using MALDI-TOF mass-spectrometry. It was shown that nucleoprotein complexes from blood of breast cancer patients contain tumor-specific proteins, such as LDOC1L, ADP/ATP translocase 3 and Lamellipodin. These data indicate, that a part of circulating extracellular DNA have tumor origin. |
Databáze: | OpenAIRE |
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