In situ ellipsometric study of surface immobilization of flagellar filaments
| Autor: | Peter Kozma, Peter Petrik, Andrea Németh, Hajnalka Jankovics, M. Fried, I. Bársony, Ferenc Vonderviszt, Sándor Kurunczi, T. Hülber, A. Sebestyén |
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| Rok vydání: | 2010 |
| Předmět: |
Chemistry
Analytical chemistry General Physics and Astronomy macromolecular substances Surfaces and Interfaces General Chemistry Condensed Matter Physics Surfaces Coatings and Films Protein filament Chemical engineering Ellipsometry Covalent bond Silanization Wafer Absorption (chemistry) Layer (electronics) Biosensor |
| Zdroj: | Applied Surface Science. 257:319-324 |
| ISSN: | 0169-4332 |
| DOI: | 10.1016/j.apsusc.2010.06.095 |
| Popis: | Protein filaments composed of thousands of subunits are promising candidates as sensing elements in biosensors. In this work in situ spectroscopic ellipsometry is applied to monitor the surface immobilization of flagellar filaments. This study is the first step towards the development of layers of filamentous receptors for sensor applications. Surface activation is performed using silanization and a subsequent glutaraldehyde crosslinking. Structure of the flagellar filament layers immobilized on activated and non-activated Si wafer substrates is determined using a two-layer effective medium model that accounted for the vertical density distribution of flagellar filaments with lengths of 300–1500 nm bound to the surface. The formation of the first interface layer can be explained by the multipoint covalent attachment of the filaments, while the second layer is mainly composed of tail pinned filaments floating upwards with the free parts. As confirmed by atomic force microscopy, covalent immobilization resulted in an increased surface density compared to absorption. |
| Databáze: | OpenAIRE |
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