6 Characterization of Microorganisms by Pyrolysis Mass Spectrometry

Autor: Colin S. Gutteridge
Rok vydání: 1988
Předmět:
DOI: 10.1016/s0580-9517(08)70412-4
Popis: Publisher Summary The identification of microorganisms using conventional procedures is a highly developed routine employing a wide range of morphological, serological, nutritional and biochemical tests. The application of Py-MS to microbiology remains underdeveloped and ill-defined. The advent of low-cost instrumentation should increase the access of microbiologists to the method. Perhaps the most significant obstacles to the more widespread use of Py- MS are the lack of understanding about what pyrolysis mass spectra represent and why they can be used to differentiate organisms. Detailed studies of microbial pyrolysis products by Py-GC-MS techniques are required to identify the cell constituents that contribute to the spectra and hence the differentiation of microorganisms. In the future, Py-MS should be used in tandem with other chemotaxonomic techniques, so that an improved understanding of the chemical basis of differences between spectra can be acquired.
Databáze: OpenAIRE