Physicochemical properties of kidney-specific antigen

Autor:	R. F. Averkina
Rok vydání:	1984
Předmět:	Kidney Deoxyribonucleoprotein RNase P Chemistry Heterologous General Medicine Trypsin General Biochemistry Genetics and Molecular Biology Papain chemistry.chemical_compound medicine.anatomical_structure Biochemistry Antigen Hyaluronidase medicine medicine.drug
Zdroj:	Bulletin of Experimental Biology and Medicine. 97:82-85
ISSN:	1573-8221 0007-4888
DOI:	10.1007/bf00830251
Popis:	Using specific antirenal sera obtained from rabbits and absorbed with a mixture of extracts from heterologous organs, a specific antigen was detected in human and CBA mouse renal extracts. Its molecular weight was found to amount to about 100 000 dalton. It is salted out with ammonium sulfate at 50-70% saturation of renal extract and is destroyed on extract heating for 30 min at 75 degrees C. This antigen is sensitive to trypsin and papain but resistant to hyaluronidase. It is partially destroyed by DNase and RNase, provided the latter ones are used in comparatively high doses (1 mg per 0.3 ml extract) and exposure lasts one day. Based on the study of the physicochemical properties it is suggested that the kidney-specific antigen may be a ribonucleoprotein or a deoxyribonucleoprotein but cannot be attributed to glycoproteins.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::482a86b0e3def83543c1dd586dcea34d https://doi.org/10.1007/bf00830251 Zobrazit plný text záznamu Full text from SpringerLink