Supercritical carbon dioxide extraction of triacontanol from green tea leaves and its evaluation as an unconventional plant growth regulator for spinach tissue culture

Autor: Kriti Kumari Dubey, Shraddha Sontakke, Rekha S. Singhal, Nupur Nagavekar
Rok vydání: 2018
Předmět:
Zdroj: Biocatalysis and Agricultural Biotechnology. 16:476-482
ISSN: 1878-8181
Popis: Triacontanol (TRIA) is a naturally synthesized non-conventional plant growth regulator (PGR) that can substitute synthetic PGRs, which are deleterious for human health and environment. TRIA was isolated from green tea leaves using Soxhlet and supercritical fluid extraction (SCF). Maximum extraction of 142 mg/kg (without alkali treatment) and 179 mg/kg (with alkali treatment) was achieved using pet-ether (60–80 °C) by Soxhlet extraction for 24 h. This was used as a standard against which the yield obtained by SCF was expressed relatively. The conditions for maximum extraction of TRIA using SCF were arrived by calculating the solubility parameters of TRIA and carbon dioxide using the Hildebrandt solubility equation. The results predicted therefrom were validated experimentally. SCF at 400 bar/60 °C/180 min gave a maximum relative extraction efficiency of 349% as compared to Soxhlet extraction without alkali treatment. TRIA thus obtained was checked for its potential as a PGR for spinach tissue culture and was compared to synthetic PGRs, TRIA and Indole acetic acid (IAA-100 µM). Medium supplemented with 5 nM TRIA increased shoot characters such as total shoot length and the number of leaves. The maximum dry weight of plant was observed in 2.2 µM TRIA containing medium. Plant nutrients like chlorophyll, carotenoids and ascorbic acid were also analyzed. Maximum yield of 84 mg/100 g ascorbic acid was obtained in plantlet grown in 2.2 µM TRIA while chlorophyll and carotenoids remained unaffected. SCF extracted TRIA showed results comparable to that of standard TRIA and IAA.
Databáze: OpenAIRE
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