| Popis: |
In this study, hemocytes and fat body changes were investigated after activation the immuneresponse of the third instar larvae of Sarcophaga argyrostoma by injecting the larvaewith MDA-MB-231cancer cell lines. To investigate the changes in hemolymph and fat bodytissue, light and transmission electron microscopy (TEM) were used. Apoptosis demonstrationin the larval flesh fly after injection was immunohistochemically tracked using a markerof caspase initiator activity; namely caspase-3 antibody.The results showed remarkable changesin morphology, viability and the spreading ability of immunocompetent hemocytes andfat body cells between normal and immunized S. argyrostoma larvae. In hemocytes, multicellularaggregates were formed to entrap invading cancer cells, in addition to membrane blebbing,cytoplasm vacuolization, cell and organelle swelling, and chromatin condensation.Meanwhile, in fat body cells of injected larvae, depletion of lipid droplets, changes in the appearanceof protein granules and degrade of basement membrane overlying the fat body wereobserved. These features are typical for apoptotic and autophagal cell death which were confirmedby detection of apoptotic fat body cells after cancer cell injection using activatedcaspase-3 immunostaining. |
