Evaluation of Expression Pattern of Vascular Endothelial Growth Factor (VEGF) and Interleukin-23 (IL-23) Genes in Human Colorectal Tumors
| Autor: | Pouladi N, Feizi Mh, Leila Rostamizadeh Hs, Kafil, Hashemzadeh S, Rezaei Fm, Sakhinia E, Zaimy Ma |
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| Rok vydání: | 2018 |
| Předmět: |
Colorectal cancer
business.industry Tumor initiation medicine.disease medicine.disease_cause Vascular endothelial growth factor 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine chemistry 030220 oncology & carcinogenesis medicine Cancer research Interleukin 23 Biomarker (medicine) 030211 gastroenterology & hepatology Risk factor business Carcinogenesis Gene |
| Zdroj: | Journal of Molecular and Genetic Medicine. 12 |
| ISSN: | 1747-0862 |
| Popis: | Background: There is a very long interval between the time of tumor initiation and diagnosis of CRCs. So, by early screening by molecular markers, individuals can be diagnosed in a much more convenient time to start the therapies. Among key factors deregulated in advanced CRC, are VEGF (vascular endothelial growth factor) and interleukin-23, which have critical roles in tumor angiogenesis and growth respectively.Objectives: The main purpose of the study was to elucidate the patterns of VEGF-A and IL-23 (IL-23p19) molecular expression in CRC.Material and methods: 47 human colorectal cancer tissues and 47 matched tumor-free margin tissues were gathered during surgery. The mRNA expression levels of VEGF-A and IL-23p19 were examined by real-time quantitative polymers chain reaction (qPCR).Results: The mRNA expression levels of VEGF-A and IL-23p19 were higher in tumor tissues than in the tumor-free margin (control) (p=0.008 and p=0.002, respectively). Though, there was no important association between the mRNA expression levels of VEGF-A and IL-23p19 with clinicopathological features. There was also a positive correlation between these 2 genes expression levels.Conclusion: The analysis of IL23p19 and VEGF-A genes expression could be considered as a biomarker for screening CRC patients and are suggested to be used for diagnosis. Thus, collectively, the high expression level of VEGF-A and IL-23p19 can be considered as a risk factor for CRC carcinogenesis, contributing to high proliferation and invasive properties of the tumor. |
| Databáze: | OpenAIRE |
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