Morphological and molecular identification of Eimeria tetartooimia oocysts from a Japanese green pheasant (Galliformes; Phasianidae; Phasianus versicolor) at a zoo in Japan

Autor: Sayaka Tsuchida, Moemi Kinoshita, Kazunari Ushida, Kazumi Sasai, Makoto Matsubayashi, Tomoyuki Shibahara, Kazutoshi Takami
Rok vydání: 2021
Předmět:
Zdroj: Parasitology Research. 120:2973-2979
ISSN: 1432-1955
0932-0113
DOI: 10.1007/s00436-021-07197-6
Popis: We detected Eimeria oocysts from Japanese green pheasants (Phasianus versicolor) at a zoo in Osaka, Japan. The oocyst isolates were subspherical or ovoidal shaped and measured 17.2 (range 14.7–20.0) μm in length and 14.8 (13.3–16.7) μm in width with a length/width (L/W) ratio of 1.2 (1.0–1.4) and each had one polar granule. The oocysts lacked a residuum and micropyle. Sporocysts measured 9.8 (6.7–13.3) μm in length and 5.9 (4.7–7.3) μm in width, with a L/W ratio of 1.2 (1.1–1.4). Compared to previously published values, this strain shows morphological similarities with an isolate of E. teetartooimia from ring-necked pheasants from other countries. Phylogenetic analysis of the 18S rRNA and mitochondrial cytochrome c oxidase subunit I genes places the isolate in a clade related to chicken Eimeria spp., such as E. acervulina or E. brunetti. Although further analysis is needed, this information can be helpful for the diagnosis and determination of virulence of Eimeria spp. in pheasants.
Databáze: OpenAIRE
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