Expression of transforming growth factor-β receptor type II and tumorigenicity in human breast adenocarcinoma MCF-7 cells
| Autor: | James K V Willson, Yong Ko, Wenhui Li, Elizabeth Zborowska, Herbert D. Soule, Sunandita S. Banerji, Robert J. Pauley, Yu Liu, Michael G. Brattain, Jiurong Liang |
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| Rok vydání: | 1998 |
| Předmět: |
medicine.medical_specialty
biology Physiology Clinical Biochemistry Cell Cell Biology Transforming growth factor beta Molecular biology Fibronectin medicine.anatomical_structure Endocrinology Cell culture Tumor progression Cell surface receptor Internal medicine medicine biology.protein skin and connective tissue diseases Receptor Transforming growth factor |
| Zdroj: | Journal of Cellular Physiology. 176:424-434 |
| ISSN: | 1097-4652 0021-9541 |
| Popis: | To analyze transforming growth factor-beta (TGF-beta) response during MCF-7 cell progression, early passage (MCF-7E, 500 passage) cells were compared. MCF-7E cells showed an IC50 of approximately 10 ng/ml of TGF-beta1, whereas MCF-7L cells were insensitive. MCF-7E cells contained approximately threefold higher levels of TGF-beta receptor type II (TbetaRII) mRNA than MCF-7L, but their TbetaRI levels were similar. MCF-7E parental cells showed higher TbetaRII promoter activity than MCF-7L cells, which could be attributed to changes in Sp1 nuclear protein levels. Receptor cross-linking studies indicated that the cell surface receptor levels parallel mRNA levels in both cell lines. Limiting dilution clones of MCF-7E cells were established to determine the heterogeneity of TbetaRII expression in this cell line, and they showed varying degrees of TbetaRII expression. Fibronectin was induced at higher levels in cells expressing higher TbetaRII levels. All three TGF-beta isoforms were detected in limiting dilution clones and parental cells, but TGF-beta1 was more abundant relative to TGF-beta2 or 3, and no correlation between TGF-beta isoform profile with TGF-beta sensitivity was found. MCF-7L cells were tumorigenic and formed xenografts rapidly and progressively, whereas MCF-7E parental and limiting dilution clonal cells showed transient tumor formation followed by regression. These results indicate that decreased TbetaRII transcription in breast cancer cells leads to a loss of TbetaRII expression, resulting in cellular resistance to TGF-beta which contributes to escape from negative growth regulation and tumor progression. |
| Databáze: | OpenAIRE |
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