Effects of detraining on the temporal expression of positive and negative angioregulatory proteins in skeletal muscle of mice

Autor: Sara Olenich, I. Mark Olfert, Gerald N. Audet, Kathleen A. Roberts
Rok vydání: 2014
Předmět:
Zdroj: The Journal of Physiology. 592:3325-3338
ISSN: 0022-3751
DOI: 10.1113/jphysiol.2014.271213
Popis: Key points Skeletal muscle capillary regression is associated with elevated thrombospondin-1 and vascular endothelial growth factor protein expression with detraining. Vascular endothelial growth factor and nucleolin responses to acute exercise are blunted in the triceps surae muscles following exercise training. In the plantaris and soleus muscles, this blunted response persists up to 28 days after cessation of training. Effects of detraining on skeletal muscle microvascular density appears to be similar among skeletal muscle of varying oxidative potential and is poorly associated with expression of matrix metalloproteinases-2 and -9 and endostatin. Abstract Temporal expression of positive and negative angiogenic factors in response to detraining is poorly understood. We report the protein expression of anti-angiogenic peptides (thrombospondin-1, TSP-1; and endostatin) as well as pro-angiogenic factors (vascular endothelial growth factor, VEGF; matrix metalloproteinases-2 and -9), and nucleolin (a nuclear protein involved with synthesis and maturation of ribosomes) in response to detraining in triceps surae muscles of C57BL/6 mice. Male mice were allowed to exercise voluntarily for 21 days, and then basal and acute response to exercise were evaluated at 1, 7, 14 and 28 days detraining (D1, D7, D14, D28, respectively, n = 12/group). As seen in the D1 mice, training resulted in the increased muscle capillary-to-fibre ratio (C/F), increased maximal running time and elevated basal expression of VEGF and matrix metalloproteinase-9 (P
Databáze: OpenAIRE
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