Systematic analysis of SmWD40s, and responding of SmWD40-170 to drought stress by regulation of ABA- and H2O2-induced stomal movement in Salvia miltiorrhiza bunge
Autor: | Junfeng Niu, Bin Li, Yuanchu Liu, Shuai Liu, Ya-ping Yan, Zhezhi Wang, Ji Ma, Wen Zhou, Wen Ma |
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Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
0301 basic medicine Gene knockdown Physiology Abiotic stress fungi food and beverages Plant Science 01 natural sciences Salvia miltiorrhiza Cell biology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Guard cell Genetics Gene family Protein kinase A Abscisic acid Gene 010606 plant biology & botany |
Zdroj: | Plant Physiology and Biochemistry. 153:131-140 |
ISSN: | 0981-9428 |
Popis: | WD40 proteins play crucial roles in response to abiotic stress. By screening the genome sequences of Salvia miltiorrhiza Bunge, 225 SmWD40 genes were identified and divided into 9 subfamilies (I-IX). Physiological, biochemical, gene structure, conserved protein motif and GO annotation analyses were performed on SmWD40 family members. The SmWD40-170 was found in 110 SmWD40 genes that contain drought response elements, SmWD40-170 was one of these genes whose response in terms of expression under drought was significant. The expression of SmWD40-170 was also up-regulated by ABA and H2O2. Through observed the stomatal phenotype of SmWD40-170 transgenic lines, the stomatal closure was abolished under dehydration, ABA and H2O2 treatment in SmWD40-170 knockdown lines. Abscisic acid (ABA), as the key phytohormone, elevates reactive oxygen species (ROS) levels under drought stress. The ABA-ROS interaction mediated the generation of H2O2 and the activation of anion channel in guard cells. The osmolality alteration of guard cells further accelerated the stomatal closure. As a second messenger, nitric oxide (NO) regulated ABA signaling, the NO stimulated protein kinase activity inhibited the K+ influx which result in stomatal closure. These NO-relevant events were essential for ABA-induced stomatal closure. The reduction of NO production was also observed in the guard cells of SmWD40-170 knockdown lines. The abolished of stomatal closure attributed to the SmWD40-170 deficiency induced the reduction of NO content. In general, the SmWD40-170 is a critical drought response gene in SmWD40 gene family and regulates ABA- and H2O2-induced stomatal movement by affecting the synthesis of NO. |
Databáze: | OpenAIRE |
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