| Autor: |
Ursula Rüegsegger, Alicia M. Esteban, Michael Betz, Herbert Zuber, Walter Sidler |
| Rok vydání: |
1993 |
| Předmět: |
|
| Zdroj: |
Biological Chemistry Hoppe-Seyler. 374:435-444 |
| ISSN: |
0177-3593 |
| Popis: |
The apcC gene from Mastigocladus laminosus encodes the linker polypeptide LC8.9 located in the phycobilisome core. A T7 RNA polymerase expression system was used to express the linker polypeptide LC8.9 from M. laminosus in Escherichia coli. The apcC gene product was expressed as an inclusion body which was solubilized in a buffer containing 8M urea. Final purification was achieved by ion exchange chromatography on Fractogel TSK CM 650 (S). In addition, a method for preparative isolation of the LC8.9 linker polypeptide from M. laminosus by reverse phase chromatography is presented. Both LC8.9 isolated from M. laminosus and overexpressed in E. coli were capable of reconstituting the complex (alpha beta)3APCLC8.9. The reconstituted complex was identical to preparations isolated from M. laminosus in terms of polypeptide composition, absorption and fluorescence emission spectroscopy. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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