Engineering of a System for the Production of Mutant Human Alpha-Fetoprotein in the Methylotrophic Yeast Pichia pastoris
| Autor: | I V Churilova, A. M. Chulkin, I V Dudich, L N Semenkova, S. V. Benevolenskii, S. S. Zatsepin, E. V. Morozkina, T A Yagudin, E. V. Klyachko, E. A. Vavilova |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Glycosylation biology Saccharomyces cerevisiae Mutant biology.organism_classification Applied Microbiology and Biotechnology Biochemistry Molecular biology Yeast law.invention Pichia pastoris carbohydrates (lipids) 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry law Recombinant DNA Fermentation Pichia |
| Zdroj: | Applied Biochemistry and Microbiology. 52:170-175 |
| ISSN: | 1608-3024 0003-6838 |
| DOI: | 10.1134/s0003683816020125 |
| Popis: | A system for the production of mutant recombinant human alpha-fetoprotein (rhAFPO) lacking the glycosylation site has been engineered in the yeast Pichia pastoris. A strain of the methylotrophic yeast Pichia pastoris GS 115/pPICZ?A/rhAFP0, which produces unglycosylated rhAFPO and secretes it to the culture medium, has been constructed. Optimization and scale-up of the fermentation technology have resulted in an increase in the rhAFP0 yield to 20 mg/L. A scheme of isolation and purification of biologically active rhAFP0 has been developed. The synthesized protein has the antitumor activity, which is analogous to the activity of natural human embryonic alpha-fetoprotein. |
| Databáze: | OpenAIRE |
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