Optimized protocol for quantification of nitrite levels in brain and head kidney tissue samples in adult zebrafish v1
Autor: | Caio Maximino, Jeisiane Souza de Oliveira, Monica Gomes Lima-Maximino |
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Rok vydání: | 2018 |
Předmět: | |
Popis: | Zebrafish, and other small teleosts, are used as experimental models to evaluate human pathologies, including those linked to oxidative and nitrosative stress, inflammation, and nitric oxide signaling. The protocol presents an optimized technique to quantify nitrite levels, in zebrafish tissues, focusing on the brain and head kidney. The protocol is based on the classical Griess diazotization reaction(Griess, 1858) method. |
Databáze: | OpenAIRE |
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