Studies on the Mechanism of the Dopamine-Mediated Inhibition of Prolactin Secretion1
| Autor: | Robert M. MacLeod, Joyce E. Lehmeyer |
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| Rok vydání: | 1974 |
| Předmět: | |
| Zdroj: | Endocrinology. 94:1077-1085 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo-94-4-1077 |
| Popis: | A study of the in vitro effect of dopamine on the release of newly synthesized prolactin is reported. Pituitary glands of female rats were incubated with 4,5-3H-leucine and the radioactive prolactin present in the pituitary gland and that released into the incubation medium were measured. Incubation with 5 × l 10–7M dopamine caused an 85% decrease in prolactin release. Prior injection of the rats with perphenazine or haloperidol rendered the pituitary gland refractory to the in vitro inhibitory effect of dopamine. Although in vitro perphenazine and haloperidol had little or no effect on release of prolactin, 5 × 10–9M of these drugs directly blocked the in vitro action of dopamine on prolactin release. Phentolamine, an α-blocking agent, was partially able to block the inhibitory effect, of dopamine. Propranolol, however, was not effective. Apomorphine, an agent known to mimic the effects of dopamine, caused a significant decrease in the amount of radioactive and radioimmunoassayable prolactin released int... |
| Databáze: | OpenAIRE |
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