| Autor: |
Masaaki Takano, Takashi Kamogashira, Michiharu Sugawara |
| Rok vydání: |
1988 |
| Předmět: |
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| Zdroj: |
Journal of Fermentation Technology. 66:649-655 |
| ISSN: |
0385-6380 |
| DOI: |
10.1016/0385-6380(88)90069-6 |
| Popis: |
The development of a very sensitive and highly specific screening method for detection of new cell wall inhibitors of the fosfomycin type is described. A fosfomycin-hypersensitive mutant, f-360, was isolated from Staphylococcus aureus Newman by selection with fosfomycin, an antibiotic that inhibits synthesis of the bacterial cell wall. The mutant f-360 was 50-fold more sensitive than the parent strain to fosfomycin. The mutant was constitutive for the hexose phosphate transport system. Using the organism in screening, BA-3796, which had an antibacterial activity against mutant f-360 was found to be produced by a bacterium designated Bacillus sp. BA-3796. Starch and beef extract were the most suitable carbon and nitrogen sources for BA-3796 production and the amount of BA-3796 reached 3 g/ l at a maximum level. The purified BA-3796 was identified as α- d -glucose-l-phosphate by its various physiochemical properties. α- d -Glucose-1-phosphate showes an antibacterial activity against Staphylococci in the presence of a slight amount pf α- d -glucose-6-phosphate. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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