The Use of Callus Cultures for Searching Proteins Associated with Increased Freezing Tolerance During Cold Acclimation in Lonicera caerulea
| Autor: | Kiyoshi Masuda, Kumiko Takada, Takashi Harada, Takashi Suzuki, Hiroyuki T Imanishi |
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| Rok vydání: | 2000 |
| Předmět: | |
| Zdroj: | Plant Biotechnology. 17:21-25 |
| ISSN: | 1347-6114 1342-4580 |
| DOI: | 10.5511/plantbiotechnology.17.21 |
| Popis: | To elucidate the molecular events underlying cold acclimation in woody plants, we established an in vitro system that responds to cold treatment, using calli of blue-berried honeysuckle (Lonicera caerulea L. var. emphyllocaryx Nakai). The calli that had been exposed to low temperature at 5°C increased their cold tolerance after 8 days of the treatment, reaching a plateau on day 12. The cold-treated calli had an approximately 60% survival rate at -7.5°C, while the survival rate of non-acclimated calli at the same temperature was below 10%. The cold-treated calli lost their freezing tolerance within 2 days of incubation at 25°C. The proteins extracted from these calli were analyzed by 2-dimensional polyacrylamide gel electrophoresis. As a result, a 42kDa (pI5.4) protein was shown to accumulate in abundance during cold acclimation. The protein decreased by culture at 25°C. These results suggest that the callus of L. caerulea did cold-acclimate in response to low temperature conditions, and that it could be used for the investigation of proteins involved in the incremental development of freezing tolerance. |
| Databáze: | OpenAIRE |
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