Comparative analysis of efficiency of syngeneic and allogeneic transplantation without irradiation of the EGFP+ mice bone marrow cells with the microspectral fluorescence method

Autor: A. V. Karnaukhov, E. V. Karnaukhova, L. A. Sergievich, I. A. Manokhina, V. N. Karnaukhov, N. A. Karnaukhova, E. V. Bogdanenko
Rok vydání: 2014
Předmět:
Zdroj: Biophysics. 59:921-927
ISSN: 1555-6654
0006-3509
DOI: 10.1134/s0006350914060049
Popis: The results of experiments on syngeneic and allogeneic transplantation of the whole fraction of mice bone marrow cells without irradiation are presented. The data were obtained on the dynamic of donor cell colonization of bone marrow, spleen, thymus, and blood of the recipient mice. The degree of immunogenicity of the donor cells for both syngeneic and allogeneic transplantation of bone marrow was determined based on the method of microspectral fluorescence analysis. The low immunogenicity degree of donor cells was demonstrated for both transplantation variants within the framework of the conducted experiment. It is important to note that the allogeneic transplantation did not cause any reduction in the mean lifespan of the mice. These results, in combination with the results of our previous studies in which an increase of average lifespan by 34% was shown with syngeneic transplantation within the C57BL/6 EGFP+ strain allow one to anticipate the development of various methods of cellular therapy without risk of fatal consequences of immunological incompatibility between donor and recipient.
Databáze: OpenAIRE
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