Mineral nutrition ofGypsophila in vitroroot culture

Autor:	Corinne Fulcheri, Max Henry, Philippe Morard
Rok vydání:	1999
Předmět:	chemistry.chemical_classification Gypsophila biology Physiology Phosphorus Potassium Saponin chemistry.chemical_element biology.organism_classification Horticulture Tissue culture chemistry.chemical_compound Micropropagation chemistry Botany Gypsophila paniculata Ammonium Agronomy and Crop Science
Zdroj:	Journal of Plant Nutrition. 22:717-730
ISSN:	1532-4087 0190-4167
DOI:	10.1080/01904169909365667
Popis:	Mineral nutrition of panicled gypsophila roots (Gypsophila paniculata L.) (Caryophyllaceae) cultivated in vitro in two media with different mineral composition, a classical Gamborg's B5 medium and a modified MH3 medium, was studied over a growth cycle of 28 days, by continuous measurement of mineral element consumption, without opening the culture flasks. The comparison between the total mineral element quantities consumed from media and those absorbed by roots at harvesting showed a good correspondence, proving the validity of the technique used and the absence of insolubilized mineral forms in the in vitro culture media. The mineral element composition of the MH3 medium was found to be better suited to Gypsophila paniculata root culture since all mineral nutrient elements are supplied in adequate quantities which are non‐limiting and non‐excessive. Culture on B5 medium showed that phosphorus (P) and copper (Cu) are probably the factors limiting growth and revealed an ammonium (NH4 +)/potassium ...
Databáze:	OpenAIRE
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