Interleukin-10 inhibits the capacity of synovial macrophages to function as antigen-presenting cells
| Autor: | R. Saario, M. Möttönen, O. Lassila, Paavo Toivanen, J. Punnonen, Pia Isomäki |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
CD40
biology business.industry CD14 medicine.medical_treatment Molecular biology Interleukin 10 Granulocyte macrophage colony-stimulating factor Cytokine Rheumatology Immunology medicine biology.protein Pharmacology (medical) Tumor necrosis factor alpha business Antigen-presenting cell Interleukin 4 medicine.drug |
| Zdroj: | Rheumatology. 37:1207-1214 |
| ISSN: | 1460-2172 |
| DOI: | 10.1093/rheumatology/37.11.1207 |
| Popis: | SUMMARY Objective. We have investigated the eVects of interleukin (IL)-10, IL-4+ granulocyte/macrophage colony-stimulating factor (GM-CSF ) and tumour necrosis factor alpha (TNF-a) on the phenotype and antigen-presenting capacity of synovial fluid (SF ) macrophages from patients with rheumatoid arthritis. Methods. The eVects of IL-4, IL-10, GM-CSF and TNF-a on the expression of surface antigens on SF macrophages were studied using flow cytometry. The eVects of these cytokines on the capacity of SF macrophages to activate T cells was investigated using the allogeneic mixed lymphocyte reaction (MLR). Results. IL-10 reduced the expression of CD40, CD86 and HLA-DR, and increased the expression of CD14, on SF macrophages. IL-10 had no eVect on the expression of CD80. Importantly, these eVects of IL-10 on the phenotype of SF macrophages appear to have functional consequences, because cells incubated with IL-10 had a significantly reduced capacity to activate T cells in MLR. The eVects of IL-4, GM-CSF and TNF-a were generally opposite to those observed in response to IL-10. IL-4 + GM-CSF, a combination of cytokines known to induce diVerentiation of dendritic cells, increased the expression of CD40, CD80 and CD86, and decreased the expression of CD14 on SF macrophages. Accordingly, IL-4 + GM-CSF increased the capacity of SF macrophages to activate T cells in MLR. IL-10 inhibited the eVects of IL-4 + GM-CSF on SF macrophages. Conclusions. IL-10 inhibits the antigen-presenting capacity of SF macrophages, which further emphasizes the antiinflammatory potential of IL-10 in RA. Importantly, IL-10 is able to downregulate the APC function of SF macrophages even when they are eYciently activated. |
| Databáze: | OpenAIRE |
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