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The development of capillary zone electrophoresis (CZE) for routine screening of black reactive dyes and black acid dyes, isolated from cotton and wool materials, is described. Detection was based on UV absorption. The electrolyte solution used was 3-(cyclohexylamino)-1-propanesulphonic acid buffer (pH 10.8), which was chosen to maintain the current at a low level under high voltages. Pretreatment of the cotton and wool samples involved extraction with NaOH or NH 3 , respectively. With the CZE technique the dyes were detected at very low concentration levels. The dye components were identified by using a newly developed marker technique. The marker components for the calculations were UV-absorbing phenylacetic acid, benzoic acid and meso -2,3-diphenylsuccinic acid. The marker technique proved effective in determining the electrophoretic mobilities of the analytes, since the relative standard deviations of the migration indices and the electrophoretic mobilities for the analytes were below 0.6%. |
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