The influences of a novel anti-adhesion device, thermally cross-linked gelatin film on peritoneal dissemination of tumor cells: The in vitro and in vivo experiments using murine carcinomatous peritonitis models
| Autor: | Akeo Hagiwara, Hiroe Miyamoto, Naoto Saitoh, Hiroyuki Tsujimoto, Yuki Ozamoto, Tsunehito Horii, Toshitaka Takagi, Joe Ueda |
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| Rok vydání: | 2017 |
| Předmět: |
Scaffold
Pathology medicine.medical_specialty Materials science Regeneration (biology) Biomedical Engineering Peritonitis Tumor cells medicine.disease In vitro 030218 nuclear medicine & medical imaging Biomaterials 03 medical and health sciences 0302 clinical medicine In vivo 030220 oncology & carcinogenesis medicine Gelatin film Preclinical imaging |
| Zdroj: | Journal of Biomedical Materials Research Part B: Applied Biomaterials. 106:2122-2130 |
| ISSN: | 1552-4973 |
| Popis: | To create anti-adhesive materials to be more effective and safer, we developed a thermally cross-linked gelatin film that showed superior anti-adhesive effects with excellent peritoneal regeneration. However, it may act as a convenient scaffold for tumor cell growth, thereby accelerating peritoneal dissemination when used in surgery for abdominal tumors. In this study, we tried to clarify this issue using mouse carcinomatous peritonitis models. First, we examined the in vitro tumor cell growth of mouse B16 melanoma or Colon26 cells on the gelatin film or the conventional hyarulonate/carboxymethylcellulose film. Tumor cell growth on each film was significantly lower than that of the control (no film). Next, we conducted the following in vivo experiments: After the parietal peritoneum was partially removed and covered with each film or without any film, mice were inoculated intraperitoneally with B16 melanoma or Colon26/Nluc cells expressing NanoLuc luciferase gene. At 7 days after the operation, we measured the weight of B16 melanoma tumors or the NanoLuc activity of Colon26/Nluc cells using in vivo imaging at the injured sites. There were no significant differences in the weight of the tumors and the NanoLuc activity among the three groups. We also observed the survival time of mice receiving the same operation and treatments. There was no significant difference in the survival time among the three groups. These results suggest that the gelatin film will likely not accelerate peritoneal dissemination as a convenient scaffold for tumor cell growth when used in surgery for abdominal tumors. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2122-2130, 2018. |
| Databáze: | OpenAIRE |
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