Prevalence and Diversity of Cag (PAI) in Helicobacter pylori: Study on Gastric Ulcer
| Autor: | Hossein Abbasinia, Firouzeh Morshedzadeh, Davood Zaeifi |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty medicine.diagnostic_test biology business.industry Rapid urease test Virulence Locus (genetics) Helicobacter pylori biology.organism_classification Gastroenterology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine 030220 oncology & carcinogenesis Internal medicine Biopsy medicine CagA Primer (molecular biology) business Gene |
| Zdroj: | Journal of Gastrointestinal & Digestive System. |
| ISSN: | 2161-069X |
| DOI: | 10.4172/2161-069x.1000564 |
| Popis: | Background: Frequency of H. Pylori in northern part of Iran within normal population is about 90% which is high, particular marker in order to diagnose patients with higher risk of peptic ulcer disease progression would be helpful for clinical practice. The purpose of this study was to evaluate the variable locus of CagA gene in virulence form. Methods: For this aim 200 types of biopsy infected and positive urease test and 20 samples of healthy people biopsies in order for control were provided. After DNA purification, the variety of locus in CagA gene was confirmed by glmM, SSA and CagV primers. Results: The results showed that 142 typical were positive for CagA. CagV primer used to variety fixed. The CagV gene exposed a special genetic variety in the 800 bp, 825 bp, 850 bp, 900 bp areas. Conclusions: The result of study on this locus suggested that variation of CagV could be consider as a paradigm in intensity and distribution of the gastric ulcer disease and key marker to prevent progression or inhibition of the progress in gastrointestinal disease. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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