Siglec-10 expression is up-regulated in activated human CD4+ T cells
| Autor: | Esther Bandala-Sanchez, Alana M. Neale, Naiara G. Bediaga, Gaetano Naselli, Leonard C. Harrison |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.diagnostic_test CD52 Chemistry T cell Immunology SIGLEC RNA General Medicine respiratory system Cell biology Flow cytometry 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Downregulation and upregulation medicine Immunology and Allergy Receptor Homeostasis 030215 immunology |
| Zdroj: | Human Immunology. 81:101-104 |
| ISSN: | 0198-8859 |
| DOI: | 10.1016/j.humimm.2020.01.009 |
| Popis: | Most sialic acid-binding immunoglobulin-like lectins (Siglecs) suppress immune cell function but are expressed at low levels on human T cells. We found that soluble CD52 inhibited T cell signalling by ligating Siglec-10, but the presence of Siglec-10 on human T cells has been questioned. To address this concern, we examined the expression of Siglec-10 at the RNA and protein level in human CD4+ T cells. Analysis by RNAseq, qPCR and flow cytometry demonstrated that, in contrast to other Siglecs, after activation of CD4+ T cells Siglec-10 was selectively upregulated in a subset of cells also high for CD52 expression. This observation is consistent with a homeostatic role for Siglec-10 in human CD4+ T cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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