Determination of the Antiretroviral Drug Zidovudine in Diluted Alkaline Electrolyte by Adsorptive Stripping Voltammetry at the Mercury Film Electrode

Autor:	Ricardo Q. Aucélio, Nicolás A. Rey, Arnaldo Aguiar Castro, Eliane Monsores Miguel, Percio A. M. Farias
Rok vydání:	2011
Předmět:	Detection limit Psychiatry and Mental health Zidovudine Chromatography Chemistry Adsorptive stripping voltammetry medicine Lamivudine Electrolyte Dropping mercury electrode Didanosine Voltammetry medicine.drug
Zdroj:	American Journal of Analytical Chemistry. :223-234
ISSN:	2156-8278 2156-8251
DOI:	10.4236/ajac.2011.22027
Popis:	This paper describes a stripping method for the determination of zidovudine at the submicromolar concentration levels. This method is based on the controlled adsorptive accumulation of zidovudine at the thin-film mercury electrode, followed by a linear-sweep stripping voltammetry measurement of the surface species. Optimal experimental conditions include a NaOH solution of 2.0 × 10–3 mol●L–1 (sup-porting electrolyte), an accumulation potential of –0.30 V and a scan rate of 100 mV?s–1. The response of zidovudine is linear over the concentration range 0.01 - 0.08 ppm. After an accumulation time of 5 minutes, the detection limit was found to be 0.67 ppb (2.5 × 10–9 mol●L–1). More convenient methods to measure zidovudine concentration in the presence of the didanosine, acyclovir, nevirapine, lamivudine, and efavirenz, were also investigated. The presence of zidovudine together with ATP or ssDNA demonstrates the utility of this method.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::471ec251e01129fb8d42ac9e54026275 https://doi.org/10.4236/ajac.2011.22027 Zobrazit plný text záznamu