Long-term maintenance of a microfluidic 3D human liver sinusoid
| Autor: | William J. McCarty, Osman Berk Usta, Abhinav Bhushan, Rohit Jindal, Shyam Sundhar Bale, Martin L. Yarmush, Ljupcho Prodanov, Inna Golberg, Manjunath Hegde |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Liver sinusoid Human liver Chemistry Microfluidics Albumin Bioengineering Long term maintenance Applied Microbiology and Biotechnology Cell biology In vitro model 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Sinusoid Porous membrane Immunology medicine Biotechnology |
| Zdroj: | Biotechnology and Bioengineering. 113:241-246 |
| ISSN: | 0006-3592 |
| Popis: | The development of long-term human organotypic liver-on-a-chip models for successful prediction of toxic response is one of the most important and urgent goals of the NIH/DARPA's initiative to replicate and replace chronic and acute drug testing in animals. For this purpose, we developed a microfluidic chip that consists of two microfluidic chambers separated by a porous membrane. The aim of this communication is to demonstrate the recapitulation of a liver sinusoid-on-a-chip, using human cells only for a period of 28 days. Using a step-by-step method for building a 3D microtissue on-a-chip, we demonstrate that an organotypic in vitro model that reassembles the liver sinusoid microarchitecture can be maintained successfully for a period of 28 days. In addition, higher albumin synthesis (synthetic) and urea excretion (detoxification) were observed under flow compared to static cultures. This human liver-on-a-chip should be further evaluated in drug-related studies. |
| Databáze: | OpenAIRE |
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