Localized Rac Activation Dynamics Visualized in Living Cells

Autor:	Martin A. Schwartz, Gary M. Bokoch, Sarah Slabaugh, Chester E. Chamberlain, Vadim Kraynov, Klaus M. Hahn
Rok vydání:	2000
Předmět:	Multidisciplinary Guanosine RAC1 Guanosine triphosphate Biology Subcellular localization Cell biology Cell membrane chemistry.chemical_compound medicine.anatomical_structure chemistry Cell polarity medicine Signal transduction Actin
Zdroj:	Science. 290:333-337
ISSN:	1095-9203 0036-8075
DOI:	10.1126/science.290.5490.333
Popis:	Signaling proteins are thought to be tightly regulated spatially and temporally in order to generate specific and localized effects. For Rac and other small guanosine triphosphatases, binding to guanosine triphosphate leads to interaction with downstream targets and regulates subcellular localization. A method called FLAIR (fluorescence activation indicator for Rho proteins) was developed to quantify the spatio-temporal dynamics of the Rac1 nucleotide state in living cells. FLAIR revealed precise spatial control of growth factor–induced Rac activation, in membrane ruffles and in a gradient of activation at the leading edge of motile cells. FLAIR exemplifies a generally applicable approach for examining spatio-temporal control of protein activity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4709f323dd8aab950fc2a8f905bf5168 https://doi.org/10.1126/science.290.5490.333 Zobrazit plný text záznamu Plný text ve formátu PDF