Effectiveness of conventional and hydrosurgical debridement methods in reducingStaphylococcus aureusinoculation of equine musclein vitro

Autor: J. M. Wilmink, D. A. Gorvy, E. M. Skärlina, Nils Fall
Rok vydání: 2014
Předmět:
Zdroj: Equine Veterinary Journal. 47:218-222
ISSN: 0425-1644
Popis: Summary Reasons for performing study The success of primary healing of equine traumatic wounds is dependent on thorough debridement. A specific hydrosurgical debridement device (VersajetTM)a is gentle to viable tissues, yet effectively removes macroscopic contaminants and debris. We wished to investigate whether it is effective in reducing bacterial burden and whether it differs from traditional methods. No previous reports compare hydrosurgical debridement and conventional wound debridement with regard to bacterial reduction from in vitro inoculated soft tissue. Objectives To assess the effectiveness of hydrosurgical debridement in reducing the Staphylococcus aureus load from in vitro inoculated equine muscle compared with conventional wound debridement methods. Study design In vitro experimental study. Methods The surface of equine masseter muscle was inoculated with a S. aureus broth and subsequently debrided using one of the following 4 methods: saline irrigation; sharp debridement; saline irrigation and sharp debridement; or hydrosurgical debridement. Tissue samples for quantitative cultures were collected before and after debridement, and the colony-forming units per gram of tissue were calculated and log transformed. The reductions in bacterial counts were analysed statistically using Wilcoxon signed-rank tests and Friedman two-way ANOVA. Results Hydrosurgical debridement was more effective than conventional debridement methods in reducing the S. aureus load (P
Databáze: OpenAIRE