Effect of Denaturating Agents on the Structural Alterations and Drug-Binding Capacity of Human and Bovine Serum Albumin

Autor:	Leszek Sułkowski, Anna Sułkowska, M. Maciążek-Jurczyk, Wiesław W. Sułkowski, J. Równicka-Zubik
Rok vydání:	2012
Předmět:	Sulindac biology Hydrochloride Serum albumin Human serum albumin Atomic and Molecular Physics and Optics Analytical Chemistry chemistry.chemical_compound Biochemistry chemistry biology.protein medicine Urea Denaturation (biochemistry) Bovine serum albumin Guanidine Spectroscopy medicine.drug
Zdroj:	Spectroscopy Letters. 45:520-529
ISSN:	1532-2289 0038-7010
DOI:	10.1080/00387010.2011.650342
Popis:	Sulindac (SDC) is a nonsteroidal anti-inflammatory drug, useful in the treatment of chronic and acute inflammatory conditions. The binding of SDC to human serum albumin and bovine serum albumin was studied by fluorescence spectroscopy and the results compared with those obtained for human and bovine serum albumin destabilized with urea and guanidine hydrochloride. SDC interacts with serum albumin within the hydrophobic subdomain IIA and/or IB. The analysis of the binding constants shows that the studied albumins preserve their binding capacity in the presence of destabilizing/denaturating agents. The denaturation coefficient has been calculated to estimate the degree of denaturation of protein.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::47045bc7c0880a21b5f05f05f3681cc8 https://doi.org/10.1080/00387010.2011.650342 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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