Interlaboratory Study of the IFCC Method for Alanine Aminotransferase Performed with Use of a Partly Purified Reference Material

Autor:	Ferruccio Ceriotti, J Muller, P. Arzoglou, G. Ferard, E Colinet, Françoise Schiele, J. Frei, Gérard Siest, H Brettschneider, D H Calam
Rok vydání:	1992
Předmět:	chemistry.chemical_classification Chromatography Pig heart biology Catalytic concentration Biochemistry (medical) Clinical Biochemistry Enzyme Biochemistry chemistry biology.protein Specific activity Alanine aminotransferase Bovine serum albumin
Zdroj:	Clinical Chemistry. 38:2365-2371
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/38.12.2365
Popis:	We present the results of a study on performance of a reference material for alanine aminotransferase (ALT, EC 2.6.1.2) and the corresponding IFCC-approved method in an interlaboratory trial involving 13 laboratories. The ALT material was partly purified from pig heart (specific activity, 150 kU/g) and was essentially free of six potentially contaminating enzyme activities, including aspartate aminotransferase (EC 2.6.1.1). The partly purified ALT was lyophilized in a triethanolamine-buffered matrix, pH 6.4, containing bovine serum albumin and saccharose. Under these conditions, the predicted yearly loss of activity was 0.02% at 4 degrees C and < 0.01% at -20 degrees C. The final blank-corrected results of the accepted set of data gave a mean (SD) of 128.5 (5.1) U/L. The among-laboratory SD was 4.6 U/L and the within-laboratory SD was 2.0 U/L. The certified ALT catalytic concentration in the reconstituted material was 129 U/L with a 0.95 confidence interval of +/- 4 U/L.
Databáze:	OpenAIRE
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