Quisqualis indica Improves Benign Prostatic Hyperplasia by Regulating Prostate Cell Proliferation and Apoptosis
| Autor: | Hyo-Jung Kwun, Charith Ub Wijerathne, Young-Won Seo, Hye-Yun Jeong, Hwa-Young Son, Young-Suk Won, Og-Sung Moon, Ji-Won Song, Sung-Hum Yeon, Jong-Hwan Lim, Hee-Seon Park |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Testosterone propionate medicine.medical_specialty Pharmaceutical Science urologic and male genital diseases 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Prostate Internal medicine LNCaP Medicine Testosterone Pharmacology business.industry General Medicine Hyperplasia medicine.disease Androgen receptor Prostate-specific antigen 030104 developmental biology Endocrinology medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Dihydrotestosterone business medicine.drug |
| Zdroj: | Biological & Pharmaceutical Bulletin. 40:2125-2133 |
| ISSN: | 1347-5215 0918-6158 |
| DOI: | 10.1248/bpb.b17-00468 |
| Popis: | Quisqualis indica (QI) has been used for treating disorders such as stomach pain, constipation, and digestion problem. This study was aimed to evaluate the therapeutic efficacy of QI extract on treating benign prostatic hyperplasia (BPH) in LNCaP human prostate cancer cell line and a testosterone-induced BPH rat model. LNCaP cells were treated with QI plus testosterone propionate (TP), and androgen receptor (AR) and prostate specific antigen (PSA) expression levels were assessed by Western blotting. To induce BPH, the rats were subjected to a daily subcutaneous injection of TP (3 mg/kg) for 4 weeks. The rats in treatment group were orally gavaged with QI (150 mg/kg) together with the TP injection. In-vitro studies showed that TP-induced increases in AR and PSA expression in LNCaP cells were reduced by QI treatment. In BPH-model rats, the prostate weight, testosterone in serum, dihydrotestosterone (DHT) concentration and 5α-reductase type 2 mRNA expression in prostate tissue were significantly reduced following the treatment with QI. TP-induced prostatic hyperplasia and the expression of proliferating cell nuclear antigen (PCNA) and cyclin D1 were significantly attenuated in QI-treated rats. In addition, QI induced apoptosis by up-regulating caspase-3 and -9 activity and decreasing the B-cell lymphoma 2 (Bcl-2)/Bcl-2-associated X protein (Bax) ratio in prostate tissues of BPH rats. Further investigation showed that TP-induced activation of AKT and glycogen synthase kinase 3β (GSK3β) was reduced by QI administration. Therefore, our findings suggest that QI attenuates the BPH state in rats through anti-proliferative and pro-apoptotic activities and might be useful in the clinical treatment of BPH. |
| Databáze: | OpenAIRE |
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