Heterologous immunoassay for screening macrolide antibiotics residues in milk based on the monoclonal antibody of tylosin

Autor:	Jian Ping Wang, Ju Xiang Liu, Jian Kang Zhang, Yong Hua Qi
Rok vydání:	2013
Předmět:	Chromatography medicine.diagnostic_test Chemistry medicine.drug_class Metabolite Immunology Heterologous Tylosin Monoclonal antibody chemistry.chemical_compound Antigen Biochemistry Immunoassay medicine Tilmicosin Agronomy and Crop Science Hapten Food Science
Zdroj:	Food and Agricultural Immunology. 24:419-431
ISSN:	1465-3443 0954-0105
DOI:	10.1080/09540105.2012.705820
Popis:	In this study, tylosin (TYL) was derivatized with 4-aminophenylacetic acid to synthesise a new hapten and the hapten was used to produce the monoclonal antibody. The obtained antibody simultaneously recognised TYL, tilmicosin, acetylisovaleryltylosin and the metabolite of TYL (desmycosin) with cross-reactivities of 100%, 62%, 97% and 93%, respectively. After evaluation of two coating antigens, a heterologous competitive indirect enzyme linked immunosorbent assay was developed to determine the four analytes in milk simultaneously. The limits of detection for the four analytes were in the range of 1.5–3.1 ng mL−1. The recoveries from fortified milk were in the range of 76.3–97.4% with coefficients of variation of 5.3–15.7%.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::46ebbfae255181b42c364a495a318751 https://doi.org/10.1080/09540105.2012.705820 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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