Development of In Vitro Matured/In Vitro Fertilized Bovine Oocytes In A Simple Defined (KSOM) Medium
| Autor: | J. R. Giles, Xiangzhong Yang, Robert H. Foote, B. K. Yang |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Platelet-derived growth factor
Future studies biology business.industry Embryo In vitro Biotechnology Andrology chemistry.chemical_compound Chemically defined medium medicine.anatomical_structure chemistry embryonic structures medicine biology.protein Animal Science and Zoology Bovine embryo Blastocyst business Platelet-derived growth factor receptor |
| Zdroj: | Journal of Reproduction and Development. 41:213-218 |
| ISSN: | 1348-4400 0916-8818 |
| DOI: | 10.1262/jrd.41.213 |
| Popis: | The experiments reported here take advantage of the large number of in vitro matured and in vitro fertilized (IVM/IVF) bovine oocytes which can be produced, permitting the design of controlled experiments to establish a simple defined medium for the study of early embryo requirements and to further test this medium as a component of co-culture. A total of 1386 IVM/IVF oocytes were used to compare a simple medium (KSOM) with complex culture conditions used successfully for culture of bovine embryos. All experiments were extensively replicated factorials. In Experiment 1, KSOM was equivalent to the complex Menezo B2 medium in producing blastocysts from IVM/IVF produced embryos and was superior to Menezo B2 medium when both were used with buffalo rat liver cells (BRLC), yielding 25% vs 8% blastocysts, respectively (P |
| Databáze: | OpenAIRE |
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