| Popis: |
A general method has been developed for determining the number of molecules per asymmetric unit, Za, of a protein crystal. The method, volume-specific amino acid analysis, relies on the direct measurement of the number of protein molecules in a crystal as normalized by the crystal volume. The method is accurate, straightforward to implement and sensitive enough for application to small (0.002 m3) crystals. Although the procedures described here are specific to proteins, extension to nucleic acid crystals should be possible. |
Plný text