Rearing of Culex spp. and Aedes spp. Mosquitoes
| Autor: | Laura D. Kramer, Michael A. Schmid, Eva Harris, Mary A. Franke, Elizabeth B. Kauffman, Anne F. Payne |
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| Rok vydání: | 2017 |
| Předmět: |
Larva
Strategy and Management Mechanical Engineering fungi Yellow fever Metals and Alloys Zoology Japanese encephalitis Biology medicine.disease medicine.disease_cause Population density Industrial and Manufacturing Engineering Dengue fever parasitic diseases medicine Saint Louis encephalitis Chikungunya Vector (molecular biology) |
| Zdroj: | BIO-PROTOCOL. 7 |
| ISSN: | 2331-8325 |
| DOI: | 10.21769/bioprotoc.2542 |
| Popis: | Mosquito-transmitted pathogens cause major public health problems and contribute substantially to the global burden of disease. Aedes mosquitoes transmit dengue, Zika, yellow fever, and Chikungunya viruses; Culex mosquitoes transmit West Nile, Japanese encephalitis, and Saint Louis encephalitis viruses, among others. Experiments utilizing laboratory-reared colonized mosquitoes can address many issues such as vector biology, vector competence, vector-pathogen interaction, and vector control. The establishment of healthy and standardized mosquito colonies requires generation and implementation of protocols, attention to detail, and an understanding of the factors that affect mosquito fitness, such as temperature and humidity, nutrient quality and availability, population density, blood feeding and mating behavior, and egg-laying requirements. Here, we present a standard protocol for the rearing of Culex spp. and Aedes spp. mosquitoes and maintenance of the mosquito colony. |
| Databáze: | OpenAIRE |
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