| Autor: |
R.A. Flavell, G.C. Grosveld, F.G. Grosveld, R. Bernards, J.M. Kooter, E. De Boer, P.F.R. Little |
| Rok vydání: |
1979 |
| Předmět: |
|
| DOI: |
10.1016/b978-0-12-604450-8.50012-2 |
| Popis: |
Publisher Summary This chapter focuses on the structure and expression of normal and abnormal globin genes. Most mammalian genes are present as a single copy per haploid genome and, hence, comprise only about one part in 106 of the total nuclear DNA. This fact impeded work on single-copy genes, but recently recombinant DNA technology and sensitive gene mapping has led to the elucidation of the structure of a number of eukaryotic genes. The chapter examines the structure of the rabbit β-globin gene. The initial approach was to construct a detailed physical map of the rabbit β-globin gene using the blotting-filter hybridization approach. This showed that the β-globin gene consists of at least two noncontiguous blocks of coding sequences separated by an intervening sequence (IVS) of 600 base pairs (bp). The experiments showed that two intervening sequences are present in the rabbit β-globin gene, one of 126 bp in length in length present between the DNA sequences for amino acid 30 and 31 and the one already mentioned above of 573 bp long, present between the sequences coding for amino acids 104 and 105. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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