Agreement of Resting Brain Perfusion using Two Different Pseudo-Continuous Arterial Spin Labeling Sequences and the Associations with Lipid Biomarkers

Autor: Kevin Decker, Faria Sanjana, Nick Rizzi, Mary Kramer, Alexander Cerjanic, Curtis Johnson, Christopher Martens
Rok vydání: 2023
Předmět:
Zdroj: Physiology. 38
ISSN: 1548-9221
1548-9213
DOI: 10.1152/physiol.2023.38.s1.5733801
Popis: Objective: We developed a modified pseudo-continuous arterial spin labeling (pCASL) sequence for measuring cerebrovascular reactivity (CVR) to hypercapnia in humans; however, the method has yet to be validated against an established pCASL sequence for assessing brain perfusion at rest. The purpose of this project was to compare resting brain perfusion from our modified sequence (Rest-CVR) to the pCASL sequence developed by the Human Connectome Project (HCP). The secondary purpose was to determine the association of these pCASL sequences with lipid biomarkers. Methods: Resting brain perfusion was assessed using both sequences in 7 middle-aged adult participants (56 ± 5 years old) using a 64-channel head coil inside a Siemens Prisma 3T MRI scanner. The modified Rest-CVR sequence included a shorter scan time (~3 minutes) with a single post-labeling delay and duration of 1800ms, FOV= 215mm2, slice thickness 2.27mm, TR=4510ms, and TE=20ms. Blood samples were also collected to determine the association of each sequence with circulating lipid biomarkers to test the sensitivity of each sequence to clinically relevant risk factors of cerebrovascular disease. Results: Outcomes from the two pCASL sequences were significantly correlated [R2 = 0.88; F (1,5) = 35.06 p = 0.002]. However, resting brain perfusion from Rest-CVR was significantly lower than HCP [Rest-CVR: 58.84 ± 15.22 vs. HCP: 81.12 ± 12.54 ml/min/100g; p = 0.0008]. Resting brain perfusion measurements from both sequences were negatively associated with low density lipoprotein (LDL) cholesterol (HCP: r = -0.97; p = 0.0001 and Rest-CVR: r = -0.87; p = 0.008) suggesting sensitivity of both to a clinically relevant risk factor for cerebrovascular disease. However, neither sequence was associated with total cholesterol, triglycerides, or high-density lipoprotein (HDL) cholesterol (p>0.05 for all). Conclusion: Rest-CVR was determined to be a reliable measure of resting brain perfusion in that it correlated with the HCP protocol; however, the lower magnitude relative to HCP makes it a less valid approach for accurately determining brain perfusion. Resting brain perfusion from both sequences was negatively associated with LDL suggesting both are sensitive to clinically relevant outcomes. The shorter Rest-CVR sequence may be most suitable for studies in which an exact estimate of brain perfusion is not required such as when assessing a relative change to hypercapnia. Grant Support: NIH/NIGMS - P20 GM113125 This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.
Databáze: OpenAIRE
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