Slug expression inhibits calcitriol-mediated sensitivity to radiation in colorectal cancer
| Autor: | Dennis K. Watson, E. Ramsay Camp, R. Eric Moretz, Silvia G. Vaena, David T. Marshall, M Ashenafi, Cindy Wang, Victoria J. Findlay, Savannah G. Bandurraga |
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| Rok vydání: | 2013 |
| Předmět: |
Cancer Research
medicine.medical_specialty Calcitriol biology Slug Colorectal cancer fungi Cancer Vimentin Transfection biology.organism_classification medicine.disease Endocrinology Radiation sensitivity Internal medicine polycyclic compounds medicine biology.protein lipids (amino acids peptides and proteins) Epithelial–mesenchymal transition Molecular Biology medicine.drug |
| Zdroj: | Molecular Carcinogenesis. 53:E130-E139 |
| ISSN: | 0899-1987 |
| Popis: | Recently, a reciprocal relationship between calcitriol and epithelial-to-mesenchymal transition has been described. Therefore, we hypothesized that calcitriol (1α,25-dihydroxyvitamin D3) would enhance radiation sensitivity in colorectal cancer regulated by epithelial mesenchymal transition. Vitamin-D receptor, E-cadherin and vimentin protein as well as E-cadherin, Snail and Slug mRNA levels were assessed in a panel of human colorectal cancer cell lines at baseline and in response calcitriol. We defined cell lines as calcitriol sensitive based on demonstrating an enhanced epithelial phenotype with increased E-cadherin, reduced vimentin and decreased expression of Snail and Slug as well as decreased cellular migration in response to calcitriol. In calcitriol sensitive cells, including DLD-1 and HCT116, 24 h calcitriol pre-treatment enhanced the radiation sensitivity by 2.3- and 2.6-fold, respectively, at 4 Gy (P |
| Databáze: | OpenAIRE |
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