Application of HPLC-ICP-MS to speciation of cisplatin and its degradation products in water containing different chloride concentrations and in human urine
| Autor: | Gerhard Stingeder, M. Fürhacker, Robert M. Mader, Wolfgang Buchberger, Zs. Stefánka, Gunda Koellensperger, Stephan Hann |
|---|---|
| Rok vydání: | 2003 |
| Předmět: | |
| Zdroj: | J. Anal. At. Spectrom.. 18:1391-1395 |
| ISSN: | 1364-5544 0267-9477 |
| DOI: | 10.1039/b309028k |
| Popis: | Cisplatin, mono- and diaquacisplatin were measured in aquatic samples and in diluted urine of a cancer patient by HPLC-ICP-MS. On-line IDMS was applied for accurate, species unspecific quantification. Limits of detection of 0.74, 0.69 and 0.65 µg L−1 (3 s criterion) were calculated for cisplatin, monoaqua- and diaquacisplatin, respectively. Degradation kinetics of 6 × 10−6 M cisplatin were determined over a period of 48 h in solutions containing 100, 50 and 0 mg L−1 chloride, showing the suitability of the HPLC-ICP-MS method for kinetic model studies. The first order rate constants k1 of cisplatin aquation for the three chloride concentrations were 1.79 × 10−5, 1.68 × 10−5 and 2.06 × 10−5 s−1. For cisplatin anatation (second order reverse reaction), rate constants of k−1 = 6.5 × 10−3, 5.8 × 10−3 and 4.1 × 10−3 M−1 s−1 could be assessed. At low chloride levels, no equilibrium was established between cisplatin and its degradation products. It was found that the intermediately formed mono- and diaquacisplatin-products started to decay after several hours. Diluted urine of a cancer patient contained the parent drug cisplatin and a considerable fraction of highly active monoaquacisplatin, as well as several unknown platinum species. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|