ISOLATION AND CHARACTERIZATION OF HELICOBACTER PYLORI STRAINS FROM GASTRIC BIOPSIES OF ALGERIAN PATIENTS
| Autor: | Guetarni Hassina, Bensoltane Ahmed |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
biology medicine.drug_class Antibiotics Rapid urease test Helicobacter pylori bacterial infections and mycoses Isolation (microbiology) biology.organism_classification Gastroenterology General Biochemistry Genetics and Molecular Biology Melting curve analysis Microbiology Agar plate Real-time polymerase chain reaction Clarithromycin Internal medicine medicine General Agricultural and Biological Sciences medicine.drug |
| Zdroj: | OnLine Journal of Biological Sciences. 13:41-49 |
| ISSN: | 1608-4217 |
| DOI: | 10.3844/ojbsci.2013.41.49 |
| Popis: | The objective of this study is to isolate and characterize strains Helicobacetr pylori from gastric biopsies by upper gastrointestinal endoscopy. After a rapid urease test, two Helicobacter pylori strains isolated from four patients on blood agar. After extraction of DNA from these strains from homogenates of gastric biopsies, quantification by real-time PCR was performed to determine Helicobacter pylori on the one hand and sensitivity to clarithromycin species. Melting curve analyzes showed that these two strains are sensitive to the antibiotic. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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