Deoxyschisandrinmodulates synchronized Ca2+oscillations and spontaneous synaptic transmission of cultured hippocampal neurons1

Autor: Zuoping Xie, Huancong Zuo, Min Zong, Zhao-hui Sun, Min Fu, Xiangping He
Rok vydání: 2008
Předmět:
Zdroj: Acta Pharmacologica Sinica. 29:891-898
ISSN: 1745-7254
1671-4083
Popis: Aim: Deoxyschisandrin is one of the most effective composites of Schisandra chinensis , a famous Chinese medicine widely used as an antistress, anti-aging, and neurological performance-improving herb. In this study, we examined its specific mechanisms of action on cultured hippocampal neurons. Methods: Hippocampal neurons, primarily cultured for 9–11 d in vitro , were used for this study. DS were dissolved in DMSO and applied to calcium imaging and whole-cell patch clamp. Results: The application of 3 mg/L DS decreased the frequency of spontaneous and synchronous oscillations of intracellular Ca 2+ to 72%±2% (mean±SEM), and the spontaneous inhibitory postsynaptic currents to 60%±3% (mean±SEM). The inhibitory concentraton 50% (IC 50 ) for the effect of DS on calcium oscillations was 3.8 mg/L. DS also depressed the high voltage-gated Ca 2+ channel and the voltage-gated Na + channel currents at the same time point. It had no effect, however, on voltage-gated K + and spontaneous excitatory postsynaptic currents. Conclusion: DS inhibited the spontaneous and synchronous oscillations of intracellular Ca 2+ through the depression of influx of extracellular calcium and the initiation of action potential. By repressing the spontaneous neurotransmitter release, DS modulated the neuronal network activities.
Databáze: OpenAIRE
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