Determination of the Intracellular Concentrations of Metabolites in Escherichia coli Collected during the Exponential and Stationary Growth Phases using Liquid Chromatography-Mass Spectrometry
| Autor: | Changhun Park, Jinwon Lee, Youn Jin Lee, Han Bin Oh, Sang Yup Lee |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Chromatography
biology Metabolite Analytical chemistry General Chemistry medicine.disease_cause biology.organism_classification Triple quadrupole mass spectrometer chemistry.chemical_compound Metabolomics chemistry Liquid chromatography–mass spectrometry Phos medicine Escherichia coli Quantitative analysis (chemistry) Intracellular |
| Zdroj: | Bulletin of the Korean Chemical Society. 32:524-530 |
| ISSN: | 0253-2964 |
| DOI: | 10.5012/bkcs.2011.32.2.524 |
| Popis: | In the present study, we demonstrate that SRM LC-MS/MS method developed by Luo et al. (ref. 10) can be successfully applied to the quantitative analysis of intracellular metabolites in E. coli that are collected at the exponential and statio- nary growth phases. A focus is given on measuring the changes in the concentrations of intracellular metabolites in batch cultures, which were induced during both the dynamically changing exponential and stationary growth phases. The following intracellular metabolites are quantified in the exponential and stationary phases of E. coli growth, using the SRM mode of a triple quadrupole mass spectrometer: glucose-1-phosphate, fructose-1,6-bisphosphate, phos- phoenolpyruvate, pyruvate, acetyl-coenzyme A, 6-phosphogluconate, ribulose-5-phosphate, xylulose-5-phosphate, erythrose-4-phosphate. The determined intracellular metabolite concentration profiles are shown to be in a good agree- ment with the growth profiles of E. coli, which clearly indicates that SRM LC-MS/MS can be successfully used for following the metabolite changes induced at different growth stages. |
| Databáze: | OpenAIRE |
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