| Autor: |
Yiping Qi, Zheng Yuan, Dabing Zhang, Weihang Gu |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Methods in Molecular Biology ISBN: 9781493989904 |
| DOI: |
10.1007/978-1-4939-8991-1_8 |
| Popis: |
Obtaining photoperiod-sensitive genic male sterility (PGMS) lines is one of the most important steps in two-line hybrid rice breeding. Traditionally, such lines were screened and developed with a classic rice breeding system under both long-day and short-day conditions. The isolation and backcross process used for this could easily last for more than 3 years with a very low success rate. Here, we describe a straightforward method for generating csa-based PGMS lines by using the CRISPR-Cas9 technology in rice. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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