Regulation of adipose tissue metabolism during pregnancy and lactation in the ewe: the role of insulin1
| Autor: | Y. Demarne, M. J. Massoud, Ph. Guesnet |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
medicine.medical_specialty
Insulin medicine.medical_treatment Adipose tissue Lipid metabolism General Medicine White adipose tissue Biology Insulin receptor Endocrinology medicine.anatomical_structure Internal medicine Lactation Lipogenesis Genetics medicine biology.protein Lipolysis Animal Science and Zoology Food Science |
| Zdroj: | Journal of Animal Science. 69:2057-2065 |
| ISSN: | 1525-3163 0021-8812 |
| DOI: | 10.2527/1991.6952057x |
| Popis: | This study was conducted to examine the effect of insulin on lipid metabolism of adipocytes during pregnancy and lactation in ewes. During the first 3 mo of pregnancy, metabolism of adipocytes from omental adipose tissue was characterized by a high rate of de novo lipogenesis (90 to 125 nmol of acetate incorporated into lipids.2 h-1.10(6) cells-1) and a 38% reduction in response to beta-lipolytic stimulus (isoproterenol 10(-6) M). Simultaneously, there was a rise in the number of high-affinity insulin receptors (Kd = .2 nM), and insulin binding characteristics showed a decrease in the negative cooperativity phenomenon. Moreover, lipogenesis stimulated by insulin (1 mU/ml) increased in comparison with observations in nonpregnant ewes. The last third of pregnancy and early lactation were characterized by a marked fall in lipogenesis and a simultaneous increase in isoproterenol-stimulated lipolysis. During lactation, the number of total insulin receptors was decreased by 62% and insulin stimulation of lipogenesis became inefficient. Results suggest that insulin plays a direct role in adipose tissue metabolism during pregnancy. |
| Databáze: | OpenAIRE |
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