Fe(II) Complexes Containing Bioactive Ligands: Synthesis, DNA Binding, and Cleavage Studies

Autor:	H. S. Bhojya Naik, B. Sreekanth, M. C. Prabhakara, G. Krishnamurthy, T. K. Vishnuvardhan
Rok vydání:	2010
Předmět:	Gel electrophoresis Absorption spectroscopy Chemistry Base pair Intercalation (chemistry) Cleavage (embryo) Binding constant Inorganic Chemistry Metal chemistry.chemical_compound Crystallography visual_art visual_art.visual_art_medium Physical and Theoretical Chemistry DNA
Zdroj:	Synthesis and Reactivity in Inorganic, Metal-Organic, and Nano-Metal Chemistry. 40:955-962
ISSN:	1553-3182 1553-3174
Popis:	The two Fe(II) complexes of fused aromatic N and S containing ligands of the type [Fe(HMq)3] (PF6)2 [complex (1)] and [Fe(HSeq)3] (PF6)2 [complex (2)] (where HMq = 2-mercapto-4-methylquinoline and HSeq = 2-seleno-4-methylquinoline) were synthesized and structurally characterized. The DNA binding property of the complexes with calf thymus DNA has been investigated using absorption spectra, viscosity measurements, as well as thermal denaturation experiments. Intrinsic binding constant Kb has been estimated at room temperature. The absorption spectral studies indicate that the complexes intercalate between the base pairs of the CT-DNA tightly with intrinsic DNA binding constant of 1.9 × 106 M−1 for complex (1) and 2.5 × 106 M−1 for complex (2) in 5 mM Tris-HCl/50 mM NaCl buffer at pH 7.2, respectively. Detailed analysis revealed that the metal complexes intercalates into the DNA base stack as intercalator. The oxidative cleavage activity of the complexes (1) and (2) were studied by using gel electrophoresis ...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::464a0c1ee086a757610d938b27dccbf6 https://doi.org/10.1080/15533174.2010.522665 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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