Construction of a linkage map for quantitative trait loci associated with economically important traits in creeping bentgrass (Agrostis stolonifera L.)

Autor: Tifu Zhang, Min Ge, Suleiman S. Bughrara, Han Zhao, Xiaoqing Ye
Rok vydání: 2012
Předmět:
Zdroj: Euphytica. 188:347-360
ISSN: 1573-5060
0014-2336
DOI: 10.1007/s10681-012-0670-8
Popis: Creeping bentgrass (Agrostis stolonifera L.) is the most widely cultivated and high-value turfgrass species. Genetic linkage maps of creeping bentgrass were constructed for quantitative trait loci (QTL) analysis of gray snow mold (Typhula incarnata) resistance, recovery and leaf width. A segregating population of 188 pseudo-F2 progeny was developed by two-way pseudo-testcross mapping strategy. Amplified fragment length polymorphism, new developed Agrostis specific expressed sequence tag-single sequence repeat (SSR), random amplified polymorphic DNA and genomic SSR markers corresponding to DNA polymorphisms heterozygous in one parent and null in the other, were scored and placed on two separate genetic linkage maps, representing each parent. In the male parent map, 100 markers were mapped to 14 linkage groups covering a total length of 793 cM with an average interval of 8.2 cM. In the female parent map, 146 markers were clustered in another 14 linkage groups spanning 805 cM with an average distance of 5.9 cM between adjacent markers. We identified three putative QTL for leaf width and one QTL for snow mold disease resistance. The construction of a linkage map and QTL analysis are expected to facilitate the development of disease resistant creeping bentgrass cultivars by using molecular marker-assisted selection.
Databáze: OpenAIRE
Externí odkaz: