INCREASED GENERATION OF REACTIVE OXYGEN SPECIES IN ISOLATED RAT FATTY LIVER DURING POSTISCHEMIC REOXYGENATION1
| Autor: | B Santoni, Fausto Catena, Elisabetta Maiolini, Gianluigi Vendemiale, Giuseppe Cavallari, Aldo Roda, Marco Domenicali, Ignazio Grattagliano, Mauro Bernardi, Paolo Caraceni, Franco Trevisani, Antonino Cavallari, Bruno Nardo, Patrizia Pasini |
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| Rok vydání: | 2001 |
| Předmět: |
chemistry.chemical_classification
Transplantation medicine.medical_specialty Reactive oxygen species Superoxide Fatty liver Glutathione Biology medicine.disease Malondialdehyde medicine.disease_cause Lipid peroxidation chemistry.chemical_compound Endocrinology chemistry Biochemistry Internal medicine medicine Steatosis Oxidative stress |
| Zdroj: | Transplantation. 71:1816-1820 |
| ISSN: | 0041-1337 |
| DOI: | 10.1097/00007890-200106270-00018 |
| Popis: | BACKGROUND Whether fatty infiltration of the liver influences the generation of reactive oxygen species (ROS) during reperfusion is unclear. Thus, this study aimed to compare the ROS formation that occurs during postanoxic reoxygenation in isolated normal and fatty livers. METHODS Isolated livers from fed Sprague-Dawley rats with normal or fatty livers induced by a choline-deficient diet were reperfused at 37 degrees C for 60 min with an oxygenated medium containing 10 microM of lucigenin after 1 hr of warm ischemia. Superoxide anion generation was assessed by the chemiluminescence (CLS) signal emitted from the organ surface. The hepatic content of malondialdehyde (MDA) and glutathione was determined at the end of reperfusion. Tissue injury was evaluated by the liver histology and the alanine aminotransferase (ALT) release in the perfusate. RESULTS CLS started rapidly with reoxygenation and it diffused to the whole organ in both groups. However, CLS emission was significantly higher in fatty liver (after 10 min: 812.425+/-39.898 vs. 294.525+/-21.068 photons/cm2/sec; P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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