Anti-inflammatory effect of the ceramide mixture extracted from genetically modified Saccharomyces cerevisiae
| Autor: | Myeongkwan Song, Soonjo Kwon, Hyun Shik Yun, Sekyung Kim |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Ceramide biology medicine.drug_class Saccharomyces cerevisiae Biomedical Engineering Bioengineering Inflammation biology.organism_classification Applied Microbiology and Biotechnology Sphingolipid Anti-inflammatory Genetically modified organism 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine chemistry Biochemistry medicine Tumor necrosis factor alpha Viability assay medicine.symptom 030215 immunology Biotechnology |
| Zdroj: | Biotechnology and Bioprocess Engineering. 22:653-658 |
| ISSN: | 1976-3816 1226-8372 |
| Popis: | The inflammatory response is an indispensable bodily reaction, but excessive inflammation is known to result in diseases such as atopic disease, bronchitis, rheumatoid arthritis, and inflammatory bowel disease. Ceramide is the basic structure of sphingolipids and ceramides have been industrially used in functional cosmetics as anti-aging agents, as well as for moisturizing skin and calming skin irritation. It also has been recently used in medicinal fields as an anti-inflammatory as well as for atopic and skin wound healing, and for skin barrier restoration. In this study, we used genetically modified Saccharomyces cerevisiae to produce ceramides. Ceramide mixture was produced by gene manipulation that amplifies the original yeast gene. To investigate their anti-inflammatory effects, nitric oxide (NO) concentrations in cell culture supernatant were measured by using the Griess reaction and the expression levels of pro-inflammatory markers, cyclooxygenase-2 (COX-2) and tumor necrosis factor-α (TNF-α), were determined by using qRT-PCR. When cells were treated with the ceramide mixture, cell viability was not reduced, but NO production was inhibited. In addition, expressions of COX-2 and TNF-α were inhibited. Based on these results, we conclude that ceramide biosynthesized from recombinant yeast can effectively reduce the expression of inflammatory enzymes and cytokines. We expected that ceramides biosynthesized in genetically modified yeast is a novel preventive or therapeutic agent for inflammatory diseases without the risk of foreign gene introduction. |
| Databáze: | OpenAIRE |
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