Interactome Analysis Reveals Regulator of G Protein Signaling 14 (RGS14) is a Novel Calcium/Calmodulin (Ca2+/CaM) and CaM Kinase II (CaMKII) Binding Partner
| Autor: | Nicholas T. Seyfried, Serena M. Dudek, John R. Hepler, Eric B. Dammer, Devrishi Goswami, Duc M. Duong, Jenny J. Yang, Kyle J. Gerber, Paul R. Evans, Daniel Lustberg, Juan Zou, Patrick R. Griffin |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Scaffold protein Calmodulin biology Chemistry General Chemistry Biochemistry Interactome Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Regulator of G protein signaling RGS14 Ca2+/calmodulin-dependent protein kinase biology.protein Protein kinase A CAMK 030217 neurology & neurosurgery |
| Zdroj: | Journal of Proteome Research. 17:1700-1711 |
| ISSN: | 1535-3907 1535-3893 |
| Popis: | Regulator of G Protein Signaling 14 (RGS14) is a complex scaffolding protein that integrates G protein and MAPK signaling pathways. In the adult mouse brain, RGS14 is predominantly expressed in hippocampal CA2 neurons where it naturally inhibits synaptic plasticity and hippocampus-dependent learning and memory. However, the signaling proteins that RGS14 natively engages to regulate plasticity are unknown. Here, we show that RGS14 exists in a high-molecular-weight protein complex in brain. To identify RGS14 neuronal interacting partners, endogenous RGS14 immunoprecipitated from mouse brain was subjected to mass spectrometry and proteomic analysis. We find that RGS14 interacts with key postsynaptic proteins that regulate plasticity. Gene ontology analysis reveals the most enriched RGS14 interactors have functional roles in actin-binding, calmodulin(CaM)-binding, and CaM-dependent protein kinase (CaMK) activity. We validate these findings using biochemical assays that identify interactions with two previousl... |
| Databáze: | OpenAIRE |
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