| Autor: |
J. W. Futrell, P. Kane, Robert R. Bahnson, Norman Wolmark, L. D. Straw, S. Iwatuski, Mitchell C. Posner, T. Logan, John M. Kirkwood, W. Shannon, John Bryant, Marc S. Ernstoff |
| Rok vydání: |
1993 |
| Předmět: |
|
| Zdroj: |
Melanoma Research. 3:451-456 |
| ISSN: |
0960-8931 |
| Popis: |
Active specific immunotherapy for cancer often requires the use of autologous or allogeneic tumour cells as immunizing antigen. Tumours were obtained for such a protocol. However, estimation of viable cell yield from pre-processed fresh tumour mass was difficult, and initially there did not appear to be a direct relationship between pre-processed tumour mass and viable cells obtained after processing. We therefore analysed all of 293 tumour specimens processed to attempt to discern such a relationship. Of these 137 were melanoma, 14 were sarcoma, 48 were adenocarcinoma, 59 were renal cell carcinoma and 35 were classified as other. A positive correlation was found between pre-processed tumour mass and viable cell yield, with Spearman correlation values varying from r = 0.49 (adenocarcinoma) to r = 0.84 (melanoma). For all tumours the Spearman correlation was r = 0.70 (p = 0.0001). Not surprisingly, the most frequent site of removal associated with bacterial contamination was bowel. In conclusion, this study provides useful curves for predicting viable tumour cell yield from pre-processed tumour mass of given histology. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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